مجال
التميز
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تميز دراسي وبحثي
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البحوث المنشورة
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البحث (1):
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عنوان البحث:
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Inhibitory effect of visfatin and leptin on
human and rat myometrial contractility
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رابط إلى البحث:
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Click here
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تاريخ النشر:
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15 Mar 2015
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موجز عن البحث:
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Aims: The purpose of this study was to
investigate the effect of visfatin on in vitro myometrial contractility in
human and rat, and compare it to leptin.Main methods: Myometrial strips from
term pregnant women having a caesarean section or rats were dissected,
superfusedwith physiological saline and the effects of visfatin (500pM–25nM)
or leptin (1nM–1μM), on spontaneous and oxytocin-induced contractionswere
studied. After establishment of regular contractions, tissueswere incubated
for control and test response at 37 °C for 20 min, and then contractility was
assayed.Key findings: In human and rat myometrium, visfatin had similar dose
dependent effects on contractility. In the human myometrium, compared with
that of controls (100%), 10 nM produced a significant (paired t-test)
decrease in the 20 min integral of spontaneous (64 ± 8%, n = 13) and
oxytocin-induced contractions (55 ± 9%, n = 5), mean ± SEM. In rat tissue the
decrease was also significant (spontaneous, 76 ± 7%, n = 7; oxytocininduced
68±6%, n=3). Leptin at this concentration (10 nM) had no effect in rat or
human, and even at a higher concentration (1 μM) produced only a small
inhibitory effect (~ 80%) on contractions.Significance: These data are the
first to show that visfatin inhibits myometrial contractility and does so
more potently than leptin. Our data suggest that increased output of visfatin
and leptin in obese pregnant women may impair uterine contractility resulting
in an unplanned Caesarean delivery.
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البحث (2):
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عنوان البحث:
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A short review of
adipokines, smooth muscle and uterine contractility
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رابط إلى البحث:
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Click here
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تاريخ النشر:
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01 Feb 2015
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موجز عن البحث:
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Obesity is a major
health problem worldwide. The prevalence of obesity is increasing in both
developed and developing countries. In the UK, for example, 60% of adults are
overweight and 25% are obese. Obesity is associated with many pathological
complications including respiratory, cardiovascular and endocrine, but it
also affects fertility and is associated with many reproductive
complications. This has led us and others to investigate links between women
with high BMI, pregnancy outcome and uterine function. These studies in turn
have led investigators to ask how obesity can have such an impact on
reproduction and, as part of this, to consider the role of the adipokines
released from adipose tissues. Our focus in this short review is on
adipokines and myometrial activity, and for completeness we overview their
effects on other smooth muscles. To date four adipokines (leptin, visfatin,
apelin and ghrelin) have been investigated and all affect myometrial
contractility, but some more potently than others. We consider the possible
mechanisms involved in how adipokines may modify uterine contractility, and
discuss the potential impact on labor and delivery.
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المؤتمرات العلمية:
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المؤتمر (1):
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عنوان المؤتمر:
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The 8th Saudi Student Conference
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تاريخ الإنعقاد:
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31 Jan – 01
Feb 2015
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مكان
الإنعقاد:
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London, UK
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طبيعة المشاركة:
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Poster presentation
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عنوان المشاركة:
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Inhibitory
effect of visfatin and leptin on human and rat myometrial contractility
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ملخص المشاركة:
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Adipokines, active substances secreted from
adipocytes influence metabolic functions. It has been suggested that their
dysregulation could be associated with the complications of pregnancy.
Recently, there has been a progressive increase in the number of women who are
either overweight or obese with a progressive increase in Caesarean section
rates. The objective of this study was to investigate the effect of visfatin
on in vitro myometrial contractility in human and rat, and compare it to
leptin. Myometrial strips from term pregnant women having a caesarean section
or rats were dissected, superfused with physiological saline and the effects
of visfatin (500pM-25nM) or leptin (1nM-1μM), on spontaneous and
oxytocin-induced contractions were studied. After establishment of regular
contractions, tissues were incubated for control and test response at 37°C
for 20min, and then contractility was assayed. In human and rat myometrium,
visfatin had similar dose dependent effects on contractility. In the human
myometrium, compared with that of controls (100%), 10nM produced a
significant (paired t-test) decrease in the 20min integral of spontaneous
(64±8%, n=13) and oxytocin-induced contractions (55±9%, n=5), mean±SEM. In
rat tissue the decrease was also significant (spontaneous, 76±7%, n=7;
oxytocin-induced 68±6%, n=3). Leptin at this concentration (10nM) had no
effect in rat or human, and even at a higher concentration (1μM) produced
only a small inhibitory effect (~ 80%) on contractions. These data are the
first to show that visfatin inhibits myometrial contractility and does so
more potently than leptin. Our data suggest that increased output of visfatin
and leptin in obese pregnant women may impair uterine contractility resulting
in an unplanned Caesarean delivery.
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